You have isolated a gene of interest that is .922 kb. After gel
purification, you find that the concentration of your gel purified
PCR product was 15.3 ng/ul. The vector you are using is 4.112 kb
and is supplied at 50ng/μl. Using the equation previously provided
in the lab handout, calculate how much insert to add in order to
achieve a final insert:vector ratio of 3:1 in a 10ul (full)
ligation reaction (3pts). Please show work and don’t get this
wrong. Someone on Chegg already answered it wrong and wasted my
time. Thanks.