The extinction coefficient for FD&C Blue Dye #1 is 138,500
cm-L/mol at 630 nm. A chemist collected the following data at 630
nm. She also found that the absorbance of an FD&C Blue Dye #1
solution of unknown concentration was 0.161. Use your graphing
calculator to generate a standard curve from this data set, and use
it to determine the concentration of the unknown solution. Express
the answer in micromolar (notice micro means 10-6)
Concentration of Blue Dye(M) Absorbance
@ 630 nm
7.58×10-6 0.101
3.26×10-5 0.288
5.17×10-5 0.521
8.63×10-5 0.782





