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Question: The extinction coefficient for FD&C Blue Dye #1 is 138,500 cm-L/mol at 630 nm. A chemist coll…

by | Nov 28, 2023 | questions

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The extinction coefficient for FD&C Blue Dye #1 is 138,500
cm-L/mol at 630 nm. A chemist collected the following data at 630
nm. She also found that the absorbance of an FD&C Blue Dye #1
solution of unknown concentration was 0.161. Use your graphing
calculator to generate a standard curve from this data set, and use
it to determine the concentration of the unknown solution. Express
the answer in micromolar (notice micro means 10-6)

Concentration of Blue Dye(M) Absorbance

@ 630 nm

7.58×10-6 0.101

3.26×10-5 0.288

5.17×10-5 0.521

8.63×10-5 0.782

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