Problem 2. Sequence below is a representative
segment of genomic DNA (coding strand of a gene).

ATGCGTACTCCTTAA

2.1 Fill in the complementary stand of the gene
so it’s double-stranded.

2.2 Translate the gene(assuming that ATG up
front is a start codon). The amino acid sequence of the polypeptide
encoded by this gene is:

2.3 You want to mutagenize your fly stock so
you expose them to EMS. Write out the double- stranded DNA after
one round of replication in the presence of EMS. (You should get
two double-stranded segments.) Assume that every nucleotide that
can be affected is affected by EMS.

2.4 You removed EMS from fly culture. Write out
the double-stranded DNA after second round of replication
without the EMS. You should have four
double-stranded segments.

2.5 Translate the four mutagenized genes from
2.4. Write out amino acid sequence of the
product.

Mutant 1:

Mutant 2:

Mutant 3:

Mutant 4:

2.6 Compare the protein product of each mutant
gene to the original (wild type) polypeptide from
2.2. For each mutant, indicate what kind of
mutation(s) it has based on sequence: nonsense, missense,
frameshift, silent.

Mutant 1:

Mutant 2:

Mutant 3:

Mutant 4: