log MW (X-axis) 1.84 1.79 1.53 1.33 1.06 60 50 40 30 20 10 0 RF values (Y axis) Y-57.43x + 110.72 R 0.9999 23 34 50 0.5 1.5 2 (Migration of protein standard during SDS PAGE, with X-axis for log MW, and Y-axis for Migration distance as mm) According to the graph above, as the size of a protein a. Its molecular weight decreases b. Its migration distance decreases c. Its migration distance increases d. None of these The slope of the linear regression line is a. Positive b. Negative c. None of these During this run, if an unknown protein’s RF value is 40, what is its estimated molecular weight? a. 1.23 KD b. 17KD c. 21KD d. 39KD