A) E. coli is one of symbiotic microorganisms found in
the lumen of our intestines. If the concentration of
lactose inside of an E. coli cell is 8.4 mM, and if the
concentration of lactose in the intestinal lumen is .0562 mM, what
is the change in free energy of transporting 1 mole of lactose from
the lumen into the cytosol of the E. coli cell at 37 oC?
F= 96,500 J/molV, R = 8.3145 J/molK. The transmembrane electrical
potential is -60 mV (OutsideInside).
(B) The pH of the cytosol of an E. coli cell is 7.38.
The pH of the intestinal lumen is 6.82. What is the free energy of
transporting 1 mole of H+ into the E. coli cell at 37oC?
F= 96,500 J/molV, R = 8.3145 J/molK. The transmembrane electrical
potential is 60 mV (OutsideInside).
(C)What is the free energy of symporting lactose and H+ from the
intestinal lumen into the cytosol of the E. coli cell?
(D) This is an classical example of what type of transport?





